dreg56  (Danaher Inc)

Journal: International Journal of Molecular Sciences

Article Title: Neutrophil-like Monocytes Increase in Patients with Colon Cancer and Induce Dysfunctional TIGIT+ NK Cells

doi: 10.3390/ijms25158470

Figure Lengend Snippet: List of antibodies used in the study.

Article Snippet: CD62L , DREG56 , PE , Beckman Coulter.

Techniques:

Journal: NPJ Precision Oncology

Article Title: Redirecting NK cells to the lymph nodes to augment their lymphoma-targeting capacity

doi: 10.1038/s41698-024-00595-w

Figure Lengend Snippet: a CCR7, CXCR5, and CD62L expression levels (% and geometric mean fluorescence intensity (GMFI)) on ex vivo expanded human NK cells 6 hours after electroporation with one or several mRNAs ( n = 10). b Transwell migration of Sham (no mRNA) or mRNA-electroporated human NK cells against gradients of one or several of the following chemokines: CCL19 (CCR7 ligand), CCL21 (CCR7 ligand), CXCL13 (CXCR5 ligand) and CXCL12 (CXCR4 ligand) ( n = 6–10). Bars, mean. Error bars, SEM. The Wilcoxon matched‐pairs signed‐rank test comparing mRNA-electroporated vs sham-electroporated NK cells was used for statistics.

Article Snippet: Anti-CD3 (1:25; UCHT1 (561416)), anti-CD16 (1:100; 3G8 (563692)), anti-CD56 (1:50; NCAM16.2 (345811)), anti-CD19 (1:50; SJ25C1 (349449)), anti-CCR7 (1:50; 150503 (562555)), anti-CD62L (1:400; DREG56 (741843)), anti-CXCR1 (1:25; 5A12 (743421)), anti-CXCR4 (1:200; 12G5 (563924)), anti-CX 3 CR1 (1:100; 2A9-1 (565796)), anti-CCR5 (1:50; 2D7 (612808)) and BD TM CompBeads were purchased from BD Biosciences.

Techniques: Expressing, Fluorescence, Ex Vivo, Electroporation, Migration

Journal: NPJ Precision Oncology

Article Title: Redirecting NK cells to the lymph nodes to augment their lymphoma-targeting capacity

doi: 10.1038/s41698-024-00595-w

Figure Lengend Snippet: a Experimental layout for in vivo homing of adoptively infused expanded human NK cells electroporated with mRNAs coding for the mouse CCR7, CXCR5. and CD62L molecules. Created with BioRender.com. b Dot plots (pooled mice for each condition) for NK cell LN homing comparing Sham vs Ccr7/Cxcr5/Cd62L mRNA conditions and assessment of NK cell number in LNs by flow cytometry, calculated out of the total live cells ( n = 9 mice). c In vivo homing of ex vivo expanded human NK cells in several organs 18–20 hours after cell transfer into SCID/Beige mice as assessed by flow cytometry, comparing Sham vs Ccr7/Cxcr5/Cd62L mRNA conditions ( n = 9 mice). d In vivo homing of ex vivo expanded human NK cells in LNs after mRNA electroporation coding for one or several molecules compared to Sham (no mRNA) ( n = 3–6 mice) and dot plots (pooled mice for each condition) for NK cell homing comparing all mRNA conditions. Bars, mean. Error bars, SEM. The Mann–Whitney U test comparing mRNA-electroporated vs sham-electroporated NK cells was used for statistics.

Article Snippet: Anti-CD3 (1:25; UCHT1 (561416)), anti-CD16 (1:100; 3G8 (563692)), anti-CD56 (1:50; NCAM16.2 (345811)), anti-CD19 (1:50; SJ25C1 (349449)), anti-CCR7 (1:50; 150503 (562555)), anti-CD62L (1:400; DREG56 (741843)), anti-CXCR1 (1:25; 5A12 (743421)), anti-CXCR4 (1:200; 12G5 (563924)), anti-CX 3 CR1 (1:100; 2A9-1 (565796)), anti-CCR5 (1:50; 2D7 (612808)) and BD TM CompBeads were purchased from BD Biosciences.

Techniques: In Vivo, Flow Cytometry, Ex Vivo, Electroporation, MANN-WHITNEY

Journal: NPJ Precision Oncology

Article Title: Redirecting NK cells to the lymph nodes to augment their lymphoma-targeting capacity

doi: 10.1038/s41698-024-00595-w

Figure Lengend Snippet: a Ex vivo expansion and b degranulation capacity of NK cells from PB of patients with FL and healthy donors ( n = 6). c Transwell migration of Sham (no mRNA) and hCCR7 mRNA-modified NK cells against gradients of CCL19 6-8 hours after electroporation ( n = 6). d Viability and expression kinetics of CCR7, CXCR5, CD62L, and CD16 molecules on ex vivo expanded human NK cells from FL patients after mRNA electroporation compared to Sham (no mRNA) ( n = 2). e Transwell migration of Sham (no mRNA) and mRNA-electroporated NK cells from two FL patients against gradients of one or several chemokines 6–8 hours after electroporation. f Degranulation capacity of NK cells from two patients with FL against different target cell lines 24 hours after mRNA electroporation compared to Sham (no mRNA). g Representative example of degranulation capacity of ex vivo expanded human NK cells from a lymphoma patient against autologous LN fine-needle biopsy material containing primary tumor cells after electroporation with Sham, CCR7, CXCR5, CD62L or additionally CD16-158V mRNA. The Mann-Whitney U test was used for statistics in a and b .

Article Snippet: Anti-CD3 (1:25; UCHT1 (561416)), anti-CD16 (1:100; 3G8 (563692)), anti-CD56 (1:50; NCAM16.2 (345811)), anti-CD19 (1:50; SJ25C1 (349449)), anti-CCR7 (1:50; 150503 (562555)), anti-CD62L (1:400; DREG56 (741843)), anti-CXCR1 (1:25; 5A12 (743421)), anti-CXCR4 (1:200; 12G5 (563924)), anti-CX 3 CR1 (1:100; 2A9-1 (565796)), anti-CCR5 (1:50; 2D7 (612808)) and BD TM CompBeads were purchased from BD Biosciences.

Techniques: Ex Vivo, Migration, Modification, Electroporation, Expressing, MANN-WHITNEY

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: A novel mass cytometry protocol optimized for immunophenotyping of low-frequency antigen-specific T cells

doi: 10.3389/fcimb.2023.1336489

Figure Lengend Snippet: Mass cytometry panel.

Article Snippet: CD62L , 153Eu , DREG56 , Standard Biotools , 1/100.

Techniques: Mass Cytometry